Effects of ascorbic acid on in vitro culture of bovine preantral follicles.
نویسندگان
چکیده
The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Compared with control fragments, the percentage of primordial follicles was reduced (p < 0.05) and the percentage of growing follicles had increased (p < 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (p < 0.05), but not when cultures were supplemented with 25, 50 or 100 μg/ml of ascorbic acid. Ultrastructural and immunohistochemical analysis of 8 day cultured ovarian cortical fragments, however, showed the integrity and viability of follicles only when fragments were cultured in presence of 50 μg/ml of ascorbic acid. In conclusion, this study demonstrated that addition of ascorbic acid to MEM at a concentration of 50 μg/ml not only stimulates the activation of 8 day in vitro cultured cattle primordial follicles and subsequent growth of activated follicles, but also safeguards the viability of these early-stage follicles.
منابع مشابه
Effect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles
متن کامل
P-36: The Effect of Lipoic Acid on Developmental Competence of Cultured Isolated Mouse Preantral Follicles
Background: α-Lipoic acid (LA) is a cofactor in mitochondrial multienzyme complexes and is well-known for its antioxidant properties in both of in vivo and in vitro condition. Materials and Methods: Preantral follicles were isolated from 2 weeks old NMRI mice ovaries. LA at concentrations of 0 and 100 μM was supplemented to culture media (α-MEM medium supplemented with 5% FBS, 100 mlU/ml FSH, 1...
متن کاملThe Effects of Fibroblast Co-Culture and Activin A on in vitro Growth of Mouse Preantral Follicles
Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base mediu...
متن کاملIn vitro culture of bovine preantral follicles: a review
Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral ...
متن کاملAn in vitro comparative study of growth media, sera and FSH effects on the growth and maturation of Syrian mice preantral follicles and enclosed-oocytes
Developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. To accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and FSH concentrations. Six-week-old preantral follicles (95 ± 5 µm) were cultured...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zygote
دوره 20 4 شماره
صفحات -
تاریخ انتشار 2012